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KMID : 0357919750090010055
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Korean Journal of Pathology
1975 Volume.9 No. 1 p.55 ~ p.60
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Separation of Lymphocytes from Human Peripheral Blood
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Abstract
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This paper describes a technique for the isolation of lymphocytes from haparinized
human peripheral blood. When whole blood was layered on top of a mixture of Ficoll
and Isopaque and gradient centrifuged at room temperature, the cellular elements of
whole blood were divided into two main parts, a lymphocyte bone in plasma layer and a
layer consisting of sedimented granulocytes and erythrocytes at the bottom of the tube.
By this method the isolation of viable lymphocyte with 96.6% purity was possible in a
short period of time. Impurities found in the lymphocyte zone was granulocytes; 0.2%,
monocytes; 2.8%, eosinophils; 0.4%, erythrocytes; less than 5% of total cell counts
respectively.
Isolating conditions and purities together with detailed directions are presented. It is
shown in the present paper that adopting the procedure introduced a lymphocyte
suspension of extreme purity with high viability may be obtained for lymphocyte
research.
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